KL-01
In vitro kidney/liver perfusion system
- KL-01 system is suitable for perfusion examination of isolated kidney or liver from small rodents, mainly rats (mice, guinea pigs). The advantage of the bath system is that freshly removed organs can be placed in a humidity chamber with physiological temperature rather than in a liquid, as in conventional baths, so that the pressure of the liquid does not stress the organ. At the same time, the organ is not dried out under these conditions that are very similar to physiological circumstances.
- The kidney or liver can be kept alive by continuous perfusion through the prepared arteries and veins using a perfusion pump, and the organs are oxygenated by a continuous flow of carbogen through the perfusion fluid. During the experiments, the temperature of the perfusion chamber, the temperature of the perfusion buffer and the vascular pressure in the organs can be continuously monitored.
- The method is suitable for the isolated study of physiological and pathophysiological processes of the liver and kidney, for the translational development of liver and kidney transplantation methods, for the isolated study of drug and drug metabolism, bile excretion, kidney and liver toxicity, and for the analysis of the interactions of drugs during hepatic and renal excretion.
- The advantage is that the structure of the organs tested is preserved, hormonal and neural factors can be excluded and even extremely high doses can be tested without the use of live animals.
The modular design of the bath system is advantageous for quick installation and maintenance. The main units are illustrated above. No special technical background is required to assemble the units of the bath system or to replace them in case of failure. The replacement of individual units can be done at user level.
- CH1 humidification/CH2 tempering chambers: the two chambers are connected with a sim ring seal. They are replaceable in one block.
- Gas input: Connection to the nebulizer in the chamber by means of a detachable silicone tube with a connector.
- Organ tub: Replacement with fixing stud.
Perfusion solution, pressure measurement: T-joint connected to the CH2 chamber outlet stub with a silicone tube for a detachable connection.
Preparing bath system for measurement
- Perfusion solution/pressure measurement: The T-joint is connected to the stump of the CH2 chamber. T-joint vertical stem for the perfusion solution, horizontal stem for the pressure sensor.
- CH2 chamber temperature measurement: The replaceable TH2 temperature sensor is located in the middle of the chamber fluid space.
- CH1 chamber temperature measurement: The temperature measurement in the measuring chamber of the organ chamber can be carried out in three positions with the TH1 temperature sensor, depending on the type and size of the organ.
- Closing chamber/organ tube: Closing can be performed with a tight-fitting cover. The closure is justified by the thermal stabilisation of the chamber, and in the case of CH2 the opening surface is also required by the replaceability of the heat trap coil.
- For kidney preparation, a 23G cannula is inserted into the ureter, renal vein and renal artery and the kidney can be removed.
- The cannula are inserted into the appropriate parts of the perfusion system and the kidney can be placed in the perfusion chamber.
- Measuring tube: 60 x 60mm.
- For liver preparation, the 18G polyethylene cannula is inserted into the portal vein and the inferior vena cava is tied under the liver.
- The chest should be opened and another 18G cannula should be inserted into the inferior vena cava from the direction of the heart and the liver removed.
- The cannula should be inserted into the appropriate parts of the perfusion system and the liver can be placed into the perfusion chamber.
- Measuring tube: 80 x 80mm.
- TC-02 two-channel measuring and control unit is suitable for heating control of enclosed fluid spaces, small animal warming blocks (e.g. isolated organ water baths, mouse, rat, rabbit operating and examination tables, etc.). Its single-current heating makes it an excellent thermostat for auxiliary units of measuring systems with high input resistance amplifiers (e.g. extra/intracellular, ECG, EGIG measurements).
- The dual cycle control optimised the heating time and minimised thermal overshoot. Thanks to the PI control principle, the instrument maintains the set temperature with an accuracy of 0.1°C.
- The need for a two-channel (CH1, CH2) version is described above. The operator interface of the channels is identical, therefore only the setting differences are presented below by channel.
- 0C: Digit display.
- LOW LEVEL: Liquid level signalling LED.
- OVER HEAT: Overheat signal.
- DISPLAY SELECT: Regulatory limit value / moment value display.
- OUTPUT SELECT: Thermo control/calibration output activation.
- SET: Potential meter to be controlled by limit value adjuster.
| TECHNICAL FEATURES | KL-01 |
|---|---|
| Application | In vitro kidney/liver perfusion system |
| Testable organ types | Isolated kidney or liver from small rodents (rat, mouse, guinea pig) |
| Physiological temperature humidity chamber |  |
| Fluid pressure does not stress the organ | |
| Organ does not dry out | |
| Organ is under circumstances similar to physiological conditions | |
| The organs can be kept alive by continuous perfusion through the prepared arteries and veins | |
| Perfusion pump circulates the perfusion fluid | |
| Oxygenation of organs is ensured by a continuous flow of carbogen through the perfusion fluid | |
| Temperature and vascular pressure can be continuously monitored | |
| Isolated study of physiological and pathophysiological processes in the liver and kidney | |
| Translational development of the method of liver and kidney transplantation | |
| Isolated studies of drug and drug metabolism, bile excretion, renal and hepatic toxicity | |
| Analysis of the interactions of individual active substances during hepatic and renal excretion | |
| Structure of the organ to be examined is retained | |
| Hormonal and neural factors can be excluded | |
| Extremely high doses can be tested | |
| Dimension | 200×100×150mm |
| Weight | 1kg |
| Thermostat | TC-02 External thermostat |
| Peristaltic pump (Optional) | |
| Pressure and temperature meter (Optional) | HC-B |
| TECHNICAL FEATURES | TC-02 Thermostat |
|---|---|
| Application | Thermostat |
| Temperature display | LED number display 3 digit |
| Temperature display | Semiconductor |
| Temperature control | Analogue PI control |
| Temperature setting | Analogue mode continuous |
| No. of channels | 2 independent thermostats |
| Working temperature range | Room temperature … +40°C |
| Temperature stability | ±0,01°C |
| Power input | 250W |
| Dimension | 320×350×220mm |
| Weight | 10kg (without liquid) |
| Power supply | On request: 110V or 220V, 50/60 Hz |
| TECHNICAL FEATURES | HG-B |
|---|---|
| Application | Two-channel pressure and temperature meter |
| HG Bandwidth | DC, 200Hz* |
| HG Calibration levels | GND, 1V |
| HG Bridge voltage | On demand |
| HG Offset | ±1V (100Hgmm) relative to output |
| HG Display | LED band |
| HG Input impedance | 10MΩ symmetric |
| HG Output impedance | 100Ω asymmetric |
| HG Value conversion: | 100Hgmm = 1V |
| TH Bandwidth | DC, 5Hz* |
| TH Calibration levels | GND, 45°C (450mV) |
| TH Bridge amplification | On demand |
| TH Display | LED number display 3 digits |
| TH Input impedance | 10kΩ symmetric |
| TH Output impedance | 100Ω asymmetric |
| TH Sensor type | PT1000 |
| TH value conversion | 10°C = 0.1V |
| Temperature accuracy | ±0,1°C |
| Power supply | On request: 110V or 220V, 50/60 Hz |
| *HG = pressure meter, TH temperature meter |
PURCHASING OPPORTUNITIES
- KL-01 In vitro kidney/liver perfusion system
- KL-01 In vitro kidney/liver perfusion system + TC-02 thermostat
- KL-01 In vitro kidney/liver perfusion system + TC-02 thermostat + HG-B pressure and temperature meter
- KL-01 In vitro kidney/liver perfusion system + TC-02 thermostat + HG-B pressure and temperature meter + Software
- KL-01 In vitro kidney/liver perfusion system + TC-02 thermostat + HG-B pressure and temperature meter + Software + Peristaltic pump
